https://hdl.handle.net/20.500.12294/215 Moleküler Biyoloji ve Genetik Bölümüne ait koleksiyonlar bu alt bölümde listelenir. Tue, 14 Apr 2026 14:28:12 GMT 2026-04-14T14:28:12Z https://hdl.handle.net/20.500.12294/4134 Combining chemical profiles and biological abilities of different extracts from Tanacetum nitens ( Boiss. & NoE) Grierson using network pharmacology Dall'Acqua, Stefano; Yagi, Sakina; Sut, Stefania; Uba, Abdullahi Ibrahim; Yildiztugay, Evren; Koyuncu, Ismail; Yuksekdag, Ozgur; Ponniya, Sathish Kumar M.; Jayavel, Ashokkumar; Zengin, Gokhan Tanacetum nitens ( Boiss. & No & euml;) Grierson is an aromatic perennial herb used in Turkish traditional medicine to treat headache, fever, and skin diseases. This study aimed to investigate the chemical composition, antioxidant, enzyme inhibition, and cytotoxic properties of T. nitens aerial parts. Organic solvent extracts were prepared by sequential maceration in hexane, dichloromethane, ethyl acetate, and methanol while aqueous extracts were obtained by maceration or infusion. Nuclear magnetic resonance (NMR) and LC-DAD-MS analysis allowed the identification and quantification of different phytoconstituents including parthenolide, tanacetol B, tatridin B, quinic acid derivatives, beta-sitosterol, and glycoside derivatives of quercetin and luteolin. The type and amount of these phytochemicals recovered by each solvent were variable and significant enough to impact the biological activities of the plant. Methanolic and aqueous extracts displayed the highest scavenging and ions-reducing properties while the dichloromethane and ethyl acetate extracts exerted the best total antioxidant activity and metal chelating power. Results of enzyme inhibition activity showed that the hexane, ethyl acetate, and dichloromethane extracts had comparable anti-acetylcholinesterase activity and the latter extract revealed the highest anti-butyrylcholinesterase activity. The best alpha-amylase and alpha-glucosidase inhibition activities were obtained from the hexane extract. The dichloromethane and ethyl acetate extracts exhibited the highest cytotoxic effect against the prostate carcinoma DU-145 cells. In conclusion, these findings indicated that T. nitens can be a promising source of biomolecules with potential therapeutic applications. Mon, 01 Jan 2024 00:00:00 GMT https://hdl.handle.net/20.500.12294/4134 2024-01-01T00:00:00Z https://hdl.handle.net/20.500.12294/4130 Exploring Chemical Composition, Antioxidant, Enzyme Inhibitory and Cytotoxic Properties of Glaucium acutidentatum Hausskn. & Bornm. from Turkey Flora Yagi, Sakina; Zengin, Gokhan; Uba, Abdullahi Ibrahim; Maciejewska-Turska, Magdalena; Sieniawska, Elwira; Swiatek, Lukasz; Rajtar, Barbara; Guler, Osman; Dall'Acqua, Stefano The present study was performed to determine the chemical constituents, cytotoxicity, antioxidant and enzyme inhibition activities of the aerial parts of Glaucium acutidentatum Hausskn. and Bornm. (family Papaveraceae). Methanolic and aqueous extracts were prepared by maceration, homogenizer-assisted extraction (HAE) and infusion. Results showed that the highest total phenolic and flavonoids contents were obtained from the methanol extracts obtained by HAE (53.22 +/- 0.10 mg GAE/g) and maceration (30.28 +/- 0.51 mg RE/g), respectively. The aporphine, beznyltetrahydroisoquinoline, and protopine types of Glaucium alkaloids have been tentatively identified. Among them, glaucine was identified in all extracts. Flavonoids, phenolic acids, coumarins, organic acids and fatty acids were also detected. Methanolic extract obtained using the HAE method displayed the highest anti-DPPH (41.42 +/- 0.62 mg TE/g), total antioxidant (1.20 +/- 0.17 mmol TE/g), Cu2+ (113.55 +/- 6.44 mg TE/g), and Fe3+ (74.52 +/- 4.74 mg TE/g) reducing properties. The aqueous extracts obtained by infusion and HAE methods exerted the best anti-ABTS (103.59 +/- 1.49 mg TE/g) and chelating (19.81 +/- 0.05 mg EDTAE/g) activities, respectively. Methanolic extract from HAE recorded the highest acetylcholinesterase (2.55 +/- 0.10 mg GALAE/g) and alpha-amylase (0.51 +/- 0.02 mmol ACAE/g) inhibition activities, while that obtained by maceration showed the best butyrylcholinesterase (3.76 +/- 0.31 mg GALAE/g) inhibition activity. Both extracts revealed the best tyrosinase inhibitory activity (25.15 +/- 1.00 and 26.79 +/- 2.36 mg KAE/g, p >= 0.05). G. acutidentatum maceration-derived aqueous extract showed selective anticancer activity against cells originating from human hypopharyngeal carcinoma. In conclusion, these findings indicated that G. acutidentatum is a promising source of alkaloids and phenolic compounds for variable pharmaceutical formulations. Mon, 01 Jan 2024 00:00:00 GMT https://hdl.handle.net/20.500.12294/4130 2024-01-01T00:00:00Z https://hdl.handle.net/20.500.12294/4124 Biological, computational evaluation of novel benzofuranyl derivatives as acetylcholinesterase and butyrylcholinesterase Usanmaz, Hande; Tas, Omer; Ekinci, Deniz; Uba, Abdullahi Ibrahim; Atmaca, Ufuk Aim: A highly efficient one-step method has been developed for the synthesis of benzofuranyl derivatives from 2-benzoylcyclohexane-1-carboxylic acid derivatives using chlorosulfonyl isocyanate. This novel method provides a practical, cost-effective and efficient approach. Materials & methods: The inhibitory effects of benzofuranyl derivatives on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were investigated. Ki values were determined to range from 0.009 to 0.61 mu M for AChE and 0.28 to 1.60 mu M for BChE. Molecular docking analysis provided insights into the interaction modes and binding patterns of these compounds with AChE and BChE. Conclusion: Kinetic findings of our study suggest that some of our compounds exhibited more effective low micromolar inhibition compared with the reference, and these derivatives could be to design more powerful agents. Mon, 01 Jan 2024 00:00:00 GMT https://hdl.handle.net/20.500.12294/4124 2024-01-01T00:00:00Z https://hdl.handle.net/20.500.12294/4123 Unveiling the potential antibacterial mechanism of Melaleuca cajuputi leaf extract by cell morphology studies and molecular docking analysis Isah, Musa; Wan Abdul Wahab, Wan-Nor-Amilah; Abdullah, Hasmah; Jamil, Shajarahtunnur; Sul'ain, Mohd Dasuki; Uba, Abdullahi Ibrahim; Zengin, Gokhan; Lahiri, Dibyajit; Edinur, Hisham Atan; Wan Ishak, Wan Rosli The antimicrobial properties of the Melaleuca cajuputi plant have been documented. However, the underlying antimicrobial mechanisms remain relatively unexplored. Thus, this study aimed to investigate the antibacterial effects of M. cajuputi leaf extract against selected bacterial strains and unveil the potential antibacterial mechanisms of the most potent sub-fraction through time-kill assay, cell morphology studies, and molecular docking analysis. The fractions and sub-fractions were obtained from the methanolic extract of M. cajuputi leaf by bioassay-guided fractionation. The antibacterial activity was tested against Staphylococcus aureus, Streptococcus agalactiae, Klebsiella pneumoniae, and Escherichia coli using broth microdilution assay. The most potent sub-fraction, Melaleuca fraction 2d (MF2d), demonstrated remarkable antibacterial activity with MIC values ranging from 0.063 to 0.25 mg/mL and induced significant cellular damage against the tested bacteria. The chemical characterization of the most potent sub-fraction (MF2d) from methanolic extract of M. cajuputi leaf identified five (5) compounds with 2-isopropyl-10-methyl phenanthrene (83.09%) as the major component. In-silico molecular docking analysis revealed that all the docked ligands showed strong binding propensity towards target bacterial proteins, including DNA gyrase (PDB ID: 1ZI0), dihydropteroate synthase (PDB ID: 1AD1), and D-alanyl transferase (PDB ID: 6O93) with the binding energy ranging from - 6.0 to - 8.4 kcal/mol. The overall findings demonstrated the potential of the M. cajuputi plant as a valuable source of novel antibacterial agents. Mon, 01 Jan 2024 00:00:00 GMT https://hdl.handle.net/20.500.12294/4123 2024-01-01T00:00:00Z